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Plant Hormones

Overview of attention for book
Cover of 'Plant Hormones'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Real-Time Analysis of the Apical Hook Development.
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    Chapter 2 Grafting with Arabidopsis thaliana.
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    Chapter 3 Tips and Tricks for Exogenous Application of Synthetic Post-translationally Modified Peptides to Plants
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    Chapter 4 Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.
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    Chapter 5 Low-Cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.
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    Chapter 6 Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.
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    Chapter 7 High-Throughput Scoring of Seed Germination.
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    Chapter 8 Histochemical Staining of β-Glucuronidase and Its Spatial Quantification.
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    Chapter 9 Imaging TCSn::GFP, a Synthetic Cytokinin Reporter, in Arabidopsis thaliana.
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    Chapter 10 Highlighting Gibberellins Accumulation Sites in Arabidopsis thaliana Root Using Fluorescently Labeled Gibberellins.
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    Chapter 11 In Silico Methods for Cell Annotation, Quantification of Gene Expression, and Cell Geometry at Single-Cell Resolution Using 3DCellAtlas.
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    Chapter 12 Analyzing Cell Wall Elasticity After Hormone Treatment: An Example Using Tobacco BY-2 Cells and Auxin.
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    Chapter 13 FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells
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    Chapter 14 In Vivo Identification of Plant Protein Complexes Using IP-MS/MS
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    Chapter 15 Assaying Auxin Receptor Activity Using SPR Assays with F-Box Proteins and Aux/IAA Degrons.
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    Chapter 16 Studying Transcription Factor Binding to Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).
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    Chapter 17 Hormone Receptor Glycosylation.
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    Chapter 18 Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.
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    Chapter 19 High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.
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    Chapter 20 Hormone Profiling in Plant Tissues.
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    Chapter 21 Use of Xenopus laevis Oocytes to Study Auxin Transport.
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    Chapter 22 Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.
Attention for Chapter 9: Imaging TCSn::GFP, a Synthetic Cytokinin Reporter, in Arabidopsis thaliana.
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Chapter title
Imaging TCSn::GFP, a Synthetic Cytokinin Reporter, in Arabidopsis thaliana.
Chapter number 9
Book title
Plant Hormones
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6469-7_9
Pubmed ID
27864760
Book ISBNs
978-1-4939-6467-3, 978-1-4939-6469-7
Authors

Jingchun Liu, Bruno Müller

Editors

Jürgen Kleine-Vehn, Michael Sauer

Abstract

Cytokinins are classical plant hormones that control numerous developmental processes throughout the plant life cycle. Cytokinin-responsive cells activate transcription via a phospho-relay signaling network. Type-B nuclear RESPONSE REGULATOR (RR) proteins mediate transcriptional activation as the final step in the signaling cascade. They bind to promoters of immediate-early target genes via a conserved Myb-related DNA-binding domain. To monitor transcriptional activation in response to a cytokinin stimulus, we have constructed a synthetic promoter, TCS (two-component signaling sensor) that harbors the concatemerized binding motifs for activated type-B RR in an optimized configuration. Here, we describe our protocols for imaging TCSn::GFP expression in transgenic Arabidopsis plants. The use of the fluorescent reporter GFP allows the visualization of cytokinin-responding cells by fluorescent microscopy without the need for tissue processing steps, or staining reactions. This method is fast and with a low risk of artifacts. However, since cytokinin signaling integrates various environmental information including light, nutrient status, and biotic and abiotic stress, special care needs to be devoted to the control of growth conditions.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 25%
Student > Master 6 15%
Researcher 5 13%
Student > Bachelor 3 8%
Student > Doctoral Student 2 5%
Other 4 10%
Unknown 10 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 45%
Biochemistry, Genetics and Molecular Biology 10 25%
Medicine and Dentistry 1 3%
Unknown 11 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 October 2017.
All research outputs
#18,482,034
of 22,901,818 outputs
Outputs from Methods in molecular biology
#7,927
of 13,133 outputs
Outputs of similar age
#310,539
of 420,462 outputs
Outputs of similar age from Methods in molecular biology
#691
of 1,074 outputs
Altmetric has tracked 22,901,818 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,133 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.

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