Chapter title |
Assaying Auxin Receptor Activity Using SPR Assays with F-Box Proteins and Aux/IAA Degrons.
|
---|---|
Chapter number | 15 |
Book title |
Plant Hormones
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6469-7_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6467-3, 978-1-4939-6469-7
|
Authors |
Mussa Quareshy, Veselina Uzunova, Justyna M. Prusinska, Richard M. Napier |
Editors |
Jürgen Kleine-Vehn, Michael Sauer |
Abstract |
The identification of TIR1 as an auxin receptor combined with advanced biophysical instrumentation has led to the development of real-time activity assays for auxins. Traditionally, molecules have been assessed for auxinic activity using bioassays, and agrochemical compound discovery continues to be based on "spray and pray" technologies. Here, we describe the methodology behind an SPR-based assay that uses TIR1 and related F-box proteins with surface plasmon resonance spectrometry for rapid compound screening. In addition, methods for collecting kinetic binding data and data processing are given so that they may support programs for rational design of novel auxin ligands. |
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