Chapter title |
Chemically Induced Cell Cycle Arrest in Perfusion Cell Culture.
|
---|---|
Chapter number | 10 |
Book title |
Cell Cycle Synchronization
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6603-5_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6602-8, 978-1-4939-6603-5
|
Authors |
Gabor Nagy, Bence Tanczos, Eszter Fidrus, Laszlo Talas, Gaspar Banfalvi |
Editors |
Gaspar Banfalvi |
Abstract |
In contrast to most present methods, continuous imaging of live cells would require full automation in each processing step. As an integrated system that would meet all requirements does not exist, we have established a long-term scanning-perfusion platform that: (a) replaces old medium with fresh one, (b) bypasses physical contact with the cell culture during continuous cell growth, (c) provides uninterrupted photomicrography of single cells, and (d) secures near physiological conditions and sterility up to several weeks. The system was validated by synchronizing cells using serum starvation and butyrate-induced cell cycle arrest of HaCaT cells. |
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Geographical breakdown
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Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 1 | 25% |
Student > Bachelor | 1 | 25% |
Researcher | 1 | 25% |
Student > Master | 1 | 25% |
Readers by discipline | Count | As % |
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Chemical Engineering | 1 | 25% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 25% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Chemistry | 1 | 25% |