| Chapter title |
Overview of Cell Synchronization.
|
|---|---|
| Chapter number | 1 |
| Book title |
Cell Cycle Synchronization
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6603-5_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6602-8, 978-1-4939-6603-5
|
| Authors |
Gaspar Banfalvi |
| Editors |
Gaspar Banfalvi |
| Abstract |
The widespread interest in cell synchronization is maintained by the studies of control mechanism involved in cell cycle regulation. During the synchronization distinct subpopulations of cells are obtained representing different stages of the cell cycle. These subpopulations are then used to study regulatory mechanisms of the cycle at the level of macromolecular biosynthesis (DNA synthesis, gene expression, protein synthesis), protein phosphorylation, development of new drugs, etc. Although several synchronization methods have been described, it is of general interest that scientists get a compilation and an updated view of these synchronization techniques. This introductory chapter summarizes: (1) the basic concepts and principal criteria of cell cycle synchronizations, (2) the most frequently used synchronization methods, such as physical fractionation (flow cytometry, dielectrophoresis, cytofluorometric purification), chemical blockade, (3) synchronization of embryonic cells, (4) synchronization at low temperature, (5) comparison of cell synchrony techniques, (6) synchronization of unicellular organisms, and (7) the effect of synchronization on transfection. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Brazil | 2 | 2% |
| Uruguay | 1 | <1% |
| Germany | 1 | <1% |
| United Kingdom | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 98 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 28 | 27% |
| Student > Master | 18 | 17% |
| Student > Bachelor | 13 | 13% |
| Researcher | 12 | 12% |
| Student > Doctoral Student | 6 | 6% |
| Other | 11 | 11% |
| Unknown | 16 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 35 | 34% |
| Agricultural and Biological Sciences | 32 | 31% |
| Engineering | 4 | 4% |
| Chemistry | 4 | 4% |
| Medicine and Dentistry | 3 | 3% |
| Other | 9 | 9% |
| Unknown | 17 | 16% |