Chapter title |
Multiplex Quantitative Polymerase Chain Reaction Diagnostic Test for SARS-CoV-2 and Influenza A/B Viruses.
|
---|---|
Chapter number | 4 |
Book title |
Multiplex Biomarker Techniques
|
Published in |
Methods in molecular biology, January 2022
|
DOI | 10.1007/978-1-0716-2395-4_4 |
Pubmed ID | |
Book ISBNs |
978-1-07-162394-7, 978-1-07-162395-4
|
Authors |
Hawkins, Steve F C, Guest, Paul C, Hawkins, Steve F. C., Guest, Paul C., Steve F. C. Hawkins, Paul C. Guest |
Abstract |
COVID-19 disease caused by the novel SARS-CoV-2 virus represents a new challenge for healthcare systems. The molecular confirmation of infection is crucial to guide public health decision-making. This task could be made more difficult during the next influenza season. Thus, a rapid and user-friendly diagnostic test to discriminate SARS-CoV-2 from influenza viruses is urgently needed. Here, we present a multiplex quantitative polymerase chain reaction (qPCR) assay capable of distinguishing SARS-CoV-2 from influenza A and B cases. This assay benefits from the use of an inhibitor tolerant PCR mix which obviates the need for the rate-limiting extraction step, allowing for a more rapid and accurate analysis. |
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