Chapter title |
Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells.
|
---|---|
Chapter number | 25 |
Book title |
Circulating Nucleic Acids in Serum and Plasma – CNAPS IX
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-42044-8_25 |
Pubmed ID | |
Book ISBNs |
978-3-31-942042-4, 978-3-31-942044-8
|
Authors |
Anna Yu. Alekseeva, Larisa V. Kameneva, Svetlana V. Kostyuk, Natalia N. Veiko |
Editors |
Peter B. Gahan, Michael Fleischhacker, Bernd Schmidt |
Abstract |
Oxidized cell-free DNA acts as a stress signal molecule and triggers an adaptive response in human cells. Various membrane DNA recognizing receptors are known as potential sensors for such DNA fragments. In order to clarify which of these sensors are able to interact with cfDNA fragments, circulating in human blood flow in heath and disease, we studied the influence of various cfDNA types on endothelial cells. We incubated these fragments at a physiologically optimal concentration with HUVEC cells for 3-24 h and detected the expression of either TLR9 or AIM2, RIG1 and STING receptors at mRNA and protein levels. We estimated that the activation of both TLR9 and other types of intracellular receptors initiates stress signaling in the endothelium independently. Signal transduction through these receptors activates NOX4 as the main source of ROS production in HUVECs. |
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Demographic breakdown
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Other | 0 | 0% |
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