Chapter title |
Vesicular and Extra-Vesicular RNAs of Human Blood Plasma.
|
---|---|
Chapter number | 23 |
Book title |
Circulating Nucleic Acids in Serum and Plasma – CNAPS IX
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-42044-8_23 |
Pubmed ID | |
Book ISBNs |
978-3-31-942042-4, 978-3-31-942044-8
|
Authors |
Anna V. Savelyeva, Dmitriy N. Baryakin, Elena D. Chikova, Elena V. Kuligina, Vladimir A. Richter, Dmitriy V. Semenov |
Editors |
Peter B. Gahan, Michael Fleischhacker, Bernd Schmidt |
Abstract |
Human blood contains a great variety of membrane-covered RNA carrying vesicles which are spherical or tubular particles enclosed by a phospholipid bilayer. Circulating vesicles are thought to mediate cell-to-cell communication and their RNA cargo can act as regulatory molecules. In this work, we separated blood plasma of healthy donors by centrifugation and determined that vesicles precipitated at 16,000 g were enriched with CD41a, marker of platelets. At 160,000 g, the pellets were enriched with CD3 marker of T cells. To characterize the RNA-content of the blood plasma sub fractions, we performed high throughput sequencing of the RNA pelleted within vesicles at 16,000 g and 160,000 g as well as RNA remaining in the vesicle-free supernatant. We found that blood plasma sub fractions contain not only extensive set of microRNAs but also fragments of other cellular RNAs: rRNAs, tRNAs, mRNAs, lncRNAs, small RNAs including RNAs encoded by mtDNAs. Our data indicate that a variety of blood plasma RNAs circulating within vesicles as well as of extra-vesicular RNAs are comparable to the variety of cellular RNA species. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 29% |
Researcher | 2 | 12% |
Student > Bachelor | 2 | 12% |
Student > Doctoral Student | 1 | 6% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 29% |
Agricultural and Biological Sciences | 5 | 29% |
Medicine and Dentistry | 2 | 12% |
Unknown | 5 | 29% |