Chapter title |
High-Throughput and High-Sensitivity Mass Spectrometry-Based N-Glycomics of Mammalian Cells.
|
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Chapter number | 14 |
Book title |
High-Throughput Glycomics and Glycoproteomics
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6493-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6491-8, 978-1-4939-6493-2
|
Authors |
Stephanie Holst, Gabi W. van Pelt, Wilma E. Mesker, Rob A. Tollenaar, Ana I. Belo, Irma van Die, Yoann Rombouts, Manfred Wuhrer |
Editors |
Gordan Lauc, Manfred Wuhrer |
Abstract |
The current protocols for glycomic analysis of cells often require a large quantity of material (5-20 million cells). In order to analyze the N-glycosylation from small amounts of cells (≤1 million) as obtained from, for example, primary cell lines or cell sorting, and in a higher throughput approach, we set up a robust 96-well format PVDF-membrane based N-glycan release protocol followed by linkage-specific sialic acid stabilization, cleanup, and MALDI-TOF-MS analysis. We further evaluated the influence of PNGase F incubation time on the N-glycan profile. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 25% |
Lecturer | 1 | 25% |
Other | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 50% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Veterinary Science and Veterinary Medicine | 1 | 25% |