Chapter title |
Analysis of N-Glycosylation of Total Membrane Proteins.
|
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Chapter number | 15 |
Book title |
High-Throughput Glycomics and Glycoproteomics
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6493-2_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6491-8, 978-1-4939-6493-2
|
Authors |
Tamara Pavić, Olga Gornik |
Editors |
Gordan Lauc, Manfred Wuhrer |
Abstract |
Glycosylation of membrane proteins plays a crucial role in various physiological events, including intercellular recognition and intermolecular interactions on the cell surface (Gornik et al., Biochim Biophys Acta 1820:1318-1326, 2012). To study composition and function of N-glycans on membrane proteins one has to have an efficient and reproducible analytical method, which includes protein extraction and analysis of glycans. In this chapter we provide an analytical approach that includes cloud-point extraction (CPE) of total membrane proteins with the non-ionic detergent Triton X-114 and subsequent analysis of their N-glycans using hydrophilic interaction liquid chromatography (HILIC)-UPLC/HPLC. The protocol presented here can be used for parallel analysis of both membrane and intracellular proteins. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 40% |
Professor > Associate Professor | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Chemistry | 2 | 40% |
Unknown | 1 | 20% |