| Chapter title |
A Novel Method to Quantify RNA-Protein Interactions In Situ Using FMTRIP and Proximity Ligation.
|
|---|---|
| Chapter number | 12 |
| Book title |
Enhancer RNAs
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-4035-6_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4033-2, 978-1-4939-4035-6
|
| Authors |
C. Zurla, J. Jung, E. L. Blanchard, P. J. Santangelo Ph.D., P. J. Santangelo |
| Editors |
Ulf Andersson Ørom |
| Abstract |
RNA binding proteins (RBP) and small RNAs regulate the editing, localization, stabilization, translation, and degradation of ribonucleic acids (RNAs) through their interactions with specific cis-acting elements within target RNAs. Here, we describe a novel method to detect protein-mRNA interactions, which combines FLAG-peptide modified, multiply-labeled tetravalent RNA imaging probes (FMTRIPs) with proximity ligation (PLA), and rolling circle amplification (RCA). This assay detects native RNA in a sequence specific and single RNA sensitive manner, and PLA allows for the quantification and localization of protein-mRNA interactions with single-interaction sensitivity. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 18% |
| Student > Postgraduate | 2 | 18% |
| Professor > Associate Professor | 2 | 18% |
| Student > Ph. D. Student | 1 | 9% |
| Student > Doctoral Student | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Immunology and Microbiology | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Neuroscience | 1 | 9% |
| Chemistry | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 4 | 36% |