| Chapter title |
Enhancer RNAs
|
|---|---|
| Chapter number | 5 |
| Book title |
Enhancer RNAs
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-4035-6_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4033-2, 978-1-4939-4035-6
|
| Authors |
Vance, Keith W, Keith W. Vance Ph.D., Keith W. Vance |
| Editors |
Ulf Andersson Ørom |
| Abstract |
Capture Hybridization Analysis of RNA Targets (CHART) has recently been developed to map the genome-wide binding profile of chromatin-associated RNAs. This protocol uses a small number of 22-28 nucleotide biotinylated antisense oligonucleotides, complementary to regions of the target RNA that are accessible for hybridization, to purify RNAs from a cross-linked chromatin extract. RNA-chromatin complexes are next immobilized on beads, washed, and specifically eluted using RNase H. Associated genomic DNA is then sequenced using high-throughput sequencing technologies and mapped to the genome to identify RNA-chromatin associations on a large scale. CHART-based strategies can be applied to determine the nature and extent of long noncoding RNA (long ncRNA) association with chromatin genome-wide and identify direct long ncRNA transcriptional targets. |
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|---|---|---|
| United Kingdom | 1 | 25% |
| France | 1 | 25% |
| United States | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
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|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 29% |
| Student > Ph. D. Student | 2 | 14% |
| Other | 1 | 7% |
| Student > Master | 1 | 7% |
| Professor > Associate Professor | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 5 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Agricultural and Biological Sciences | 1 | 7% |
| Immunology and Microbiology | 1 | 7% |
| Neuroscience | 1 | 7% |
| Unknown | 6 | 43% |