Chapter title |
Enhancer RNAs
|
---|---|
Chapter number | 7 |
Book title |
Enhancer RNAs
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-4035-6_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4033-2, 978-1-4939-4035-6
|
Authors |
Choy, Jocelyn, Fullwood, Melissa J, Jocelyn Choy, Melissa J. Fullwood Ph.D., Melissa J. Fullwood |
Editors |
Ulf Andersson Ørom |
Abstract |
Genomic DNA is dynamically associated with protein factors and folded to form chromatin fibers. The 3-dimensional (3D) configuration of the chromatin will enable the distal genetic elements to come into close proximity, allowing transcriptional regulation. Noncoding RNA can mediate the 3D structure of chromatin. Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET) is a valuable and powerful technique in molecular biology which allows the study of unbiased, genome-wide de novo chromatin interactions with paired-end tags. Here, we describe the standard version of ChIA-PET and a Multiplex ChIA-PET version. |
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