Chapter title |
Analysis of Genotyping-by-Sequencing (GBS) Data.
|
---|---|
Chapter number | 15 |
Book title |
Plant Bioinformatics
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3167-5_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3166-8, 978-1-4939-3167-5
|
Authors |
Kagale, Sateesh, Koh, Chushin, Clarke, Wayne E, Bollina, Venkatesh, Parkin, Isobel A P, Sharpe, Andrew G, Sateesh Kagale, Chushin Koh, Wayne E. Clarke, Venkatesh Bollina, Isobel A. P. Parkin, Andrew G. Sharpe |
Editors |
David Edwards |
Abstract |
The development of genotyping-by-sequencing (GBS) to rapidly detect nucleotide variation at the whole genome level, in many individuals simultaneously, has provided a transformative genetic profiling technique. GBS can be carried out in species with or without reference genome sequences yields huge amounts of potentially informative data. One limitation with the approach is the paucity of tools to transform the raw data into a format that can be easily interrogated at the genetic level. In this chapter we describe bioinformatics tools developed to address this shortfall together with experimental design considerations to fully leverage the power of GBS for genetic analysis. |
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