| Chapter title |
Multiphoton Microscopy for Visualizing Lipids in Tissue.
|
|---|---|
| Chapter number | 9 |
| Book title |
The Wilms' Tumor (WT1) Gene
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-4023-3_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4021-9, 978-1-4939-4023-3
|
| Authors |
Martin Lee, Alan Serrels |
| Editors |
Nicholas Hastie |
| Abstract |
Visualizing the appearance of fat droplets and adipocytes in tissue can be realized using a label-free imaging method known as coherent anti-Stokes Raman spectroscopy (CARS). CARS is a nonlinear optical technique that allows label-free imaging of a material with contrast based on the same vibrational signatures of molecules found in Raman spectroscopy. CARS can be combined with other single and multiphoton imaging modes such as second harmonic generation and two-photon fluorescence to image a broad variety of biological structures.Here we describe the construction of a multiphoton microscope that will enable the study of both fluorescently labeled and unlabeled tissue. This has been used to monitor the contribution of Wt1 expressing cells towards the visceral fat depots during gestation. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 33% |
| Student > Doctoral Student | 1 | 33% |
| Unknown | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 33% |
| Physics and Astronomy | 1 | 33% |
| Unknown | 1 | 33% |