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| Chapter title |
Isolation and Colony Formation of Murine Bone and Bone Marrow Cells.
|
|---|---|
| Chapter number | 6 |
| Book title |
The Wilms' Tumor (WT1) Gene
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-4023-3_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4021-9, 978-1-4939-4023-3
|
| Authors |
Sophie McHaffie, You-Ying Chau |
| Editors |
Nicholas Hastie |
| Abstract |
Adult homeostasis is dependent on normal Wt1 expression. Loss of Wt1 expression in adult mice causes rapid loss of the mesenchymal tissues, fat and bone, amongst other phenotypes. Bone and bone marrow mesenchymal stromal cells can be studied by cell isolation and expansion. The stemness of these cells can then be characterized by carrying out a colony-forming unit-fibroblast assay and observing clonogenic capabilities. |