Chapter title |
Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions.
|
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Chapter number | 440 |
Book title |
Embryonic Stem Cell Protocols
|
Published in |
Methods in molecular biology, October 2021
|
DOI | 10.1007/7651_2021_440 |
Pubmed ID | |
Book ISBNs |
978-1-07-162436-4, 978-1-07-162437-1
|
Authors |
Isaja, Luciana, Ferriol-Laffouillere, Sofía Luján, Mucci, Sofía, Rodríguez-Varela, María Soledad, Romorini, Leonardo |
Abstract |
Human embryonic stem cells (hESCs) can differentiate into any cell lineage (pluripotency potential) derived from the three germ layers: ectoderm, mesoderm, and endoderm. Pluripotency is usually demonstrated in vitro by spontaneous differentiation of hESCs grown on a monolayer of feeder-cells using an embryoid bodies (EBs)-based method. However, currently hESCs are grown mostly using fully defined media in the absence of a feeder layer. Here we describe a EBs-based protocol that allows multilineage differentiation of hESCs and human induced pluripotent stem cells (hiPSCs) grown on feeder-free conditions. |
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