Potassium Channels

Chapter 1 Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA—Protein Interactions by Chromatin Immunoprecipitation

Chapter 2 Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries

Chapter 3 Cloning of Potassium Channel Splice Variants from Tissues and Cells

Chapter 4 Chemiluminescence assays to investigate membrane expression and clathrin-mediated endocytosis of K(ATP) channels.

Chapter 5 Investigation of K(ATP) channel endocytosis by immunofluorescence.

Chapter 6 Investigation of K(ATP) channel endocytosis and cell surface density by Biotinylation and Western blotting.

Chapter 7 Lipid microdomains and k(+) channel compartmentation: detergent and non-detergent-based methods for the isolation and characterisation of cholesterol-enriched lipid rafts.

Chapter 8 Determination of Phosphoinositide Binding to K + Channel Subunits Using a Protein—Lipid Overlay Assay

Chapter 9 Protein Complex Analysis of Native Brain Potassium Channels by Proteomics

Chapter 10 Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique.

Chapter 11 Whole-Cell Recording Using the Perforated Patch Clamp Technique

Chapter 12 Recording the Activity of ATP-Sensitive K + Channels in Open-Cell Cell-Attached Configuration

Chapter 13 Planar Patch Clamp: Advances in Electrophysiology

Chapter 14 Analysing Steroid Modulation of BK Ca Channels Reconstituted into Planar Lipid Bilayers

Chapter 15 Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly

Chapter 16 The Use of FRET Microscopy to Elucidate Steady State Channel Conformational Rearrangements and G Protein Interaction with the GIRK Channels

Chapter 17 The Voltage-Clamp Fluorometry Technique

Chapter 18 Identification of Mutations in the Kir6.2 Subunit of the K ATP Channel

Chapter 19 Modulation of Potassium Ion Channel Proteins Utilising Antibodies

Chapter 20 Fluorescence-Based Tl + −Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca 2+ −Activated K + Channel Modulators

Chapter 21 Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Opening Properties

Chapter 22 Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-Clamp Technique