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The Nucleolus

Overview of attention for book
Cover of 'The Nucleolus'

Table of Contents

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    Book Overview
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    Chapter 1 The Nucleolus
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    Chapter 2 The Nucleolus
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    Chapter 3 Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces cerevisiae
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    Chapter 4 High-Throughput Live-Cell Microscopy Analysis of Association Between Chromosome Domains and the Nucleolus in S. cerevisiae
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    Chapter 5 Quantitative Immunofluorescence Analysis of Nucleolus-Associated Chromatin
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    Chapter 6 The Nucleolus
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    Chapter 7 Purification of Crystallization-Grade RNA Polymerase I from S. cerevisiae
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    Chapter 8 The Nucleolus
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    Chapter 9 The Nucleolus
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    Chapter 10 The Nucleolus
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    Chapter 11 Metabolic Labeling in the Study of Mammalian Ribosomal RNA Synthesis
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    Chapter 12 The Nucleolus
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    Chapter 13 The Nucleolus
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    Chapter 14 Analysis of rRNA Gene Methylation in Arabidopsis thaliana by CHEF-Conventional 2D Gel Electrophoresis
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    Chapter 15 Fluorescence-Activated Nucleolus Sorting in Arabidopsis
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    Chapter 16 Purification of RNA Polymerase I-Associated Chromatin from Yeast Cells
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    Chapter 17 The Nucleolus
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    Chapter 18 The Nucleolus
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    Chapter 19 The Nucleolus
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    Chapter 20 Quantitative Proteomic Analysis of the Human Nucleolus
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    Chapter 21 Analysis of Mass Spectrometry Data for Nucleolar Proteomics Experiments
Attention for Chapter 5: Quantitative Immunofluorescence Analysis of Nucleolus-Associated Chromatin
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Chapter title
Quantitative Immunofluorescence Analysis of Nucleolus-Associated Chromatin
Chapter number 5
Book title
The Nucleolus
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3792-9_5
Pubmed ID
27576710
Book ISBNs
978-1-4939-3790-5, 978-1-4939-3792-9
Authors

Stefan Dillinger, Attila Németh, Dillinger, Stefan, Németh, Attila

Editors

Attila Németh

Abstract

The nuclear distribution of eu- and heterochromatin is nonrandom, heterogeneous, and dynamic, which is mirrored by specific spatiotemporal arrangements of histone posttranslational modifications (PTMs). Here we describe a semiautomated method for the analysis of histone PTM localization patterns within the mammalian nucleus using confocal laser scanning microscope images of fixed, immunofluorescence stained cells as data source. The ImageJ-based process includes the segmentation of the nucleus, furthermore measurements of total fluorescence intensities, the heterogeneity of the staining, and the frequency of the brightest pixels in the region of interest (ROI). In the presented image analysis pipeline, the perinucleolar chromatin is selected as primary ROI, and the nuclear periphery as secondary ROI.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 29%
Student > Ph. D. Student 2 29%
Unspecified 1 14%
Other 1 14%
Student > Master 1 14%
Other 0 0%
Readers by discipline Count As %
Medicine and Dentistry 2 29%
Agricultural and Biological Sciences 2 29%
Mathematics 1 14%
Unspecified 1 14%
Biochemistry, Genetics and Molecular Biology 1 14%
Other 0 0%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2016.
All research outputs
#20,338,537
of 22,884,315 outputs
Outputs from Methods in molecular biology
#9,920
of 13,131 outputs
Outputs of similar age
#330,764
of 393,711 outputs
Outputs of similar age from Methods in molecular biology
#1,054
of 1,471 outputs
Altmetric has tracked 22,884,315 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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