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The Nucleolus

Overview of attention for book
Cover of 'The Nucleolus'

Table of Contents

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    Book Overview
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    Chapter 1 The Nucleolus
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    Chapter 2 The Nucleolus
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    Chapter 3 Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces cerevisiae
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    Chapter 4 High-Throughput Live-Cell Microscopy Analysis of Association Between Chromosome Domains and the Nucleolus in S. cerevisiae
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    Chapter 5 Quantitative Immunofluorescence Analysis of Nucleolus-Associated Chromatin
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    Chapter 6 The Nucleolus
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    Chapter 7 Purification of Crystallization-Grade RNA Polymerase I from S. cerevisiae
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    Chapter 8 The Nucleolus
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    Chapter 9 The Nucleolus
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    Chapter 10 The Nucleolus
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    Chapter 11 Metabolic Labeling in the Study of Mammalian Ribosomal RNA Synthesis
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    Chapter 12 The Nucleolus
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    Chapter 13 The Nucleolus
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    Chapter 14 Analysis of rRNA Gene Methylation in Arabidopsis thaliana by CHEF-Conventional 2D Gel Electrophoresis
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    Chapter 15 Fluorescence-Activated Nucleolus Sorting in Arabidopsis
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    Chapter 16 Purification of RNA Polymerase I-Associated Chromatin from Yeast Cells
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    Chapter 17 The Nucleolus
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    Chapter 18 The Nucleolus
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    Chapter 19 The Nucleolus
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    Chapter 20 Quantitative Proteomic Analysis of the Human Nucleolus
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    Chapter 21 Analysis of Mass Spectrometry Data for Nucleolar Proteomics Experiments
Attention for Chapter 3: Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces cerevisiae
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Chapter title
Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces cerevisiae
Chapter number 3
Book title
The Nucleolus
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3792-9_3
Pubmed ID
27576708
Book ISBNs
978-1-4939-3790-5, 978-1-4939-3792-9
Authors

Christophe Normand, Maxime Berthaud, Olivier Gadal, Isabelle Léger-Silvestre, Normand, Christophe, Berthaud, Maxime, Gadal, Olivier, Léger-Silvestre, Isabelle

Editors

Attila Németh

Abstract

Nucleoli form around RNA polymerase I transcribed ribosomal RNA (rRNA) genes. The direct electron microscopy observation of rRNA genes after nucleolar chromatin spreading (Miller's spreads) constitutes to date the only system to quantitatively assess transcription at a single molecule level. However, the spreading procedure is likely generating artifact and despite being informative, these spread rRNA genes are far from their in vivo situation. The integration of the structural characterization of spread rRNA genes in the three-dimensional (3D) organization of the nucleolus would represent an important scientific achievement. Here, we describe a correlative light and electron microscopy (CLEM) protocol allowing detection of tagged-Pol I by fluorescent microscopy and high-resolution imaging of the nucleolar ultrastructural context. This protocol can be implemented in laboratories equipped with conventional fluorescence and electron microscopes and does not require sophisticated "pipeline" for imaging.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 2 25%
Researcher 2 25%
Student > Ph. D. Student 1 13%
Student > Bachelor 1 13%
Unknown 2 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Agricultural and Biological Sciences 2 25%
Immunology and Microbiology 1 13%
Unknown 2 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 July 2017.
All research outputs
#17,813,370
of 22,884,315 outputs
Outputs from Methods in molecular biology
#7,243
of 13,131 outputs
Outputs of similar age
#267,799
of 393,711 outputs
Outputs of similar age from Methods in molecular biology
#752
of 1,471 outputs
Altmetric has tracked 22,884,315 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,711 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.

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