Chapter title |
The Nucleolus
|
---|---|
Chapter number | 12 |
Book title |
The Nucleolus
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3792-9_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3790-5, 978-1-4939-3792-9
|
Authors |
Wang, Minshi, Pestov, Dimitri G, Minshi Wang, Dimitri G. Pestov |
Editors |
Attila Németh |
Abstract |
Assembly of eukaryotic ribosomes is an elaborate biosynthetic process that begins in the nucleolus and requires hundreds of cellular factors. Analysis of rRNA processing has been instrumental for studying the mechanisms of ribosome biogenesis and effects of stress conditions on the molecular milieu of the nucleolus. Here, we describe the quantitative analysis of the steady-state levels of rRNA precursors, applicable to studies in mammalian cells and other organisms. We include protocols for gel electrophoresis and northern blotting of rRNA precursors using procedures optimized for the large size of these RNAs. We also describe the ratio analysis of multiple precursors, a technique that facilitates the accurate assessment of changes in the efficiency of individual pre-rRNA processing steps. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 50% |
Student > Bachelor | 2 | 11% |
Student > Doctoral Student | 1 | 6% |
Student > Master | 1 | 6% |
Student > Postgraduate | 1 | 6% |
Other | 0 | 0% |
Unknown | 4 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 33% |
Agricultural and Biological Sciences | 4 | 22% |
Medicine and Dentistry | 3 | 17% |
Chemistry | 1 | 6% |
Unknown | 4 | 22% |