Chapter title |
Design, construction, and characterization methodologies for synthetic microbial consortia.
|
---|---|
Chapter number | 4 |
Book title |
Engineering and Analyzing Multicellular Systems
|
Published in |
Methods in molecular biology, April 2014
|
DOI | 10.1007/978-1-4939-0554-6_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0553-9, 978-1-4939-0554-6
|
Authors |
Bernstein HC, Carlson RP, Hans C. Bernstein, Ross P. Carlson, Bernstein, Hans C., Carlson, Ross P. |
Editors |
Lianhong Sun, Wenying Shou |
Abstract |
Engineered microbial consortia are of growing interest to a range of scientists including bioprocess engineers, systems biologists, and microbiologists because of their ability to simultaneously optimize multiple tasks, to test fundamental systems science, and to understand the microbial ecology of environments like chronic wounds. Metabolic engineering, synthetic biology, and microbial ecology provide a sound scientific basis for designing, building, and analyzing consortium-based microbial platforms.This chapter outlines strategies and protocols useful for (1) in silico network design, (2) experimental strain construction, (3) consortia culturing including biofilm growth methods, and (4) physiological characterization of consortia. The laboratory and computational methods given here may be adapted for synthesis and characterization of other engineered consortia designs. |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 35 | 100% |
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Researcher | 9 | 26% |
Student > Bachelor | 6 | 17% |
Student > Doctoral Student | 3 | 9% |
Student > Ph. D. Student | 3 | 9% |
Professor | 2 | 6% |
Other | 5 | 14% |
Unknown | 7 | 20% |
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Business, Management and Accounting | 2 | 6% |
Other | 4 | 11% |
Unknown | 8 | 23% |