Structural Proteomics

Chapter 1 The Protein Data Bank Archive

Chapter 2 Computational Methods for the Elucidation of Protein Structure and Interactions

Chapter 3 Methods for Molecular Modelling of Protein Complexes

Chapter 4 High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus

Chapter 5 Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small Scale: Application for Structural Studies

Chapter 6 Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells

Chapter 7 SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering

Chapter 8 Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification

Chapter 9 Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems

Chapter 10 Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins

Chapter 11 Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources

Chapter 12 From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.

Chapter 13 Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods

Chapter 14 Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection

Chapter 15 Cryo-Focused Ion Beam Lamella Preparation Protocol for in Situ Structural Biology

Chapter 16 Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED