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Structural Proteomics

Overview of attention for book
Cover of 'Structural Proteomics'

Table of Contents

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    Book Overview
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    Chapter 1 The Protein Data Bank Archive
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    Chapter 2 Computational Methods for the Elucidation of Protein Structure and Interactions
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    Chapter 3 Methods for Molecular Modelling of Protein Complexes
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    Chapter 4 High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus
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    Chapter 5 Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small Scale: Application for Structural Studies
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    Chapter 6 Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells
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    Chapter 7 SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering
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    Chapter 8 Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification
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    Chapter 9 Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems
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    Chapter 10 Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins
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    Chapter 11 Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources
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    Chapter 12 From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.
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    Chapter 13 Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods
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    Chapter 14 Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection
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    Chapter 15 Cryo-Focused Ion Beam Lamella Preparation Protocol for in Situ Structural Biology
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    Chapter 16 Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED
Attention for Chapter 12: From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.
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Chapter title
From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.
Chapter number 12
Book title
Structural Proteomics
Published in
Methods in molecular biology, January 2021
DOI 10.1007/978-1-0716-1406-8_12
Pubmed ID
33950393
Book ISBNs
978-1-07-161405-1, 978-1-07-161406-8
Authors

Diebolder, Christoph A, Dillard, Rebecca S, Renault, Ludovic, Christoph A. Diebolder, Rebecca S. Dillard, Ludovic Renault, Diebolder, Christoph A., Dillard, Rebecca S.

Abstract

In this chapter, we present an overview of a standard protocol to achieve structure determination at high resolution by Single Particle Analysis cryogenic Electron Microscopy using apoferritin as a standard sample. The purified apoferritin is applied to a glow-discharged support and then flash frozen in liquid ethane. The prepared grids are loaded into the electron microscope and checked for particle spreading and ice thickness. The microscope alignments are performed and the data collection session is setup for an overnight data collection. The collected movies containing two-dimensional images of the apoferritin sample are then processed to obtain a high-resolution three-dimensional reconstruction.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 33%
Student > Ph. D. Student 1 17%
Other 1 17%
Unknown 2 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 33%
Arts and Humanities 1 17%
Immunology and Microbiology 1 17%
Unknown 2 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2021.
All research outputs
#20,707,815
of 23,308,124 outputs
Outputs from Methods in molecular biology
#10,097
of 13,325 outputs
Outputs of similar age
#429,183
of 502,964 outputs
Outputs of similar age from Methods in molecular biology
#196
of 256 outputs
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So far Altmetric has tracked 13,325 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 256 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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