| Chapter title |
Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells.
|
|---|---|
| Chapter number | 5 |
| Book title |
Multipotent Stem Cells of the Hair Follicle
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3786-8_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3784-4, 978-1-4939-3786-8
|
| Authors |
Fang Liu, Haiyan Lin, Chuansen Zhang |
| Editors |
Robert M Hoffman |
| Abstract |
Tissue-engineered nerve conduits are widely used for the study of peripheral nerve injury repair. With regard to repairing long nerve defects, stem-cell-derived neurons are recommended as seed cells. As hair-follicle neural crest stem cells (hfNCSCs) are easily to be harvested from patients and have the potential to differentiate into neuronal cells, hfNCSCs-derived neurons are an ideal candidate choice. Acellular nerve grafts, a type of biological material scaffold, with intact collagen structure, with biocompatibility and less toxicity are obtained through removing live cells with 1 % lysolecithin, are also an ideal choice. In the present report, we describe a tissue-engineered nerve conduit seeded with rat hfNCSCs-derived neurons into the beagle acellular sciatic nerve scaffold. Our goal is to provide a novel engineered therapeutic for repairing peripheral nerve injury with long distance defects. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 18% |
| Researcher | 2 | 18% |
| Student > Doctoral Student | 2 | 18% |
| Student > Master | 2 | 18% |
| Unknown | 3 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 3 | 27% |
| Biochemistry, Genetics and Molecular Biology | 1 | 9% |
| Neuroscience | 1 | 9% |
| Chemistry | 1 | 9% |
| Unknown | 5 | 45% |