| Chapter title |
Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization.
|
|---|---|
| Chapter number | 11 |
| Book title |
3D Cell Culture
|
| Published in |
Methods in molecular biology, December 2010
|
| DOI | 10.1007/978-1-60761-984-0_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-60761-983-3, 978-1-60761-984-0
|
| Authors |
Rui C. Pereira, Chiara Gentili, Ranieri Cancedda, Helena S. Azevedo, Rui L. Reis |
| Abstract |
This chapter is intended to provide a summary of the current materials used in cell encapsulation technology as well as methods for evaluating the performance of cells encapsulated in a polymeric matrix. In particular, it describes the experimental procedure to prepare a hydrogel matrix based on natural polymers for encapsulating and culturing human articular chondrocytes with the interest in cartilage regeneration. Protocols to evaluate the viability, proliferation, differentiation, and matrix production of embedded cells are also described and include standard protocols such as the MTT and [3H] Thymidine assays, reverse transcription polymerase chain reaction (RT-PCR) technique, histology, and immunohistochemistry analysis. The assessment of cell distribution within the 3D hydrogel construct is also described using APoTome analysis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 5% |
| Norway | 1 | 5% |
| Unknown | 20 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 23% |
| Student > Ph. D. Student | 4 | 18% |
| Other | 2 | 9% |
| Student > Doctoral Student | 2 | 9% |
| Professor | 2 | 9% |
| Other | 4 | 18% |
| Unknown | 3 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 18% |
| Medicine and Dentistry | 3 | 14% |
| Biochemistry, Genetics and Molecular Biology | 2 | 9% |
| Computer Science | 2 | 9% |
| Materials Science | 2 | 9% |
| Other | 2 | 9% |
| Unknown | 7 | 32% |