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Xenopus Protocols

Overview of attention for book
Cover of 'Xenopus Protocols'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present, and Future
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    Chapter 2 Husbandry of Xenopus tropicalis
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    Chapter 3 Generating Diploid Embryos from Xenopus tropicalis
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    Chapter 4 Navigating the Xenopus tropicalis Genome
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    Chapter 5 Genetic Analysis of Xenopus tropicalis
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    Chapter 6 Forward Genetic Screens in Xenopus Using Transposon-Mediated Insertional Mutagenesis
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    Chapter 7 Targeted Gene Disruption with Engineered Zinc-Finger Nucleases (ZFNs).
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    Chapter 8 Reverse Genetic Studies Using Antisense Morpholino Oligonucleotides
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    Chapter 9 Chemical Genetics and Drug Discovery in Xenopus
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    Chapter 10 Maternal mRNA Knock-down Studies: Antisense Experiments Using the Host-Transfer Technique in Xenopus laevis and Xenopus tropicalis
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    Chapter 11 Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI).
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    Chapter 12 A simple method of transgenesis using I-sce I meganuclease in Xenopus.
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    Chapter 13 Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos
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    Chapter 14 Xenopus Transgenics: Methods Using Transposons
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    Chapter 15 Comparative Genomics-Based Identification and Analysis of Cis-Regulatory Elements
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    Chapter 16 Tet-On Binary Systems for Tissue-Specific and Inducible Transgene Expression
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    Chapter 17 Chromatin Immunoprecipitation Analysis of Xenopus Embryos
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    Chapter 18 Transcriptomics using next generation sequencing technologies.
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    Chapter 19 Databases of Gene Expression in Xenopus Development
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    Chapter 20 Investigating Alternative RNA Splicing in Xenopus
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    Chapter 21 Immunoisolation of Protein Complexes from Xenopus
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    Chapter 22 Complementary Proteomic Analysis of Protein Complexes
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    Chapter 23 Antibody development and use in chromogenic and fluorescent immunostaining.
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    Chapter 24 Multicolor Fluorescent In Situ mRNA Hybridization (FISH) on Whole Mounts and Sections.
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    Chapter 25 Methods to Analyze microRNA Expression and Function During Xenopus Development.
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    Chapter 26 A Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos.
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    Chapter 27 Microscopy Tools for Quantifying Developmental Dynamics in Xenopus Embryos
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    Chapter 28 Mathematical Modeling of Gene Regulatory Networks in Xenopus Development
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    Chapter 29 Stem-Cell-Like Embryonic Explants to Study Cardiac Development
  31. Altmetric Badge
    Chapter 30 Xenopus Protocols
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    Chapter 31 On-Line Resources for Xenopus
Attention for Chapter 18: Transcriptomics using next generation sequencing technologies.
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Chapter title
Transcriptomics using next generation sequencing technologies.
Chapter number 18
Book title
Xenopus Protocols
Published in
Methods in molecular biology, September 2012
DOI 10.1007/978-1-61779-992-1_18
Pubmed ID
22956096
Book ISBNs
978-1-61779-991-4, 978-1-61779-992-1
Authors

Lee-Liu D, Almonacid LI, Faunes F, Melo F, Larrain J, Dasfne Lee-Liu, Leonardo I. Almonacid, Fernando Faunes, Francisco Melo, Juan Larrain, Lee-Liu, Dasfne, Almonacid, Leonardo I., Faunes, Fernando, Melo, Francisco, Larrain, Juan

Abstract

Next generation sequencing technologies may now be applied to the study of transcriptomics. RNA-Seq or RNA sequencing employs high-throughput sequencing of complementary DNA fragments delivering a transcriptional profile. In this chapter, we aim to provide a starting point for Xenopus researchers planning on starting an RNA-Seq transcriptomics study. We begin by providing a section on template isolation and library preparation. The next section comprises the main bioinformatics procedures that need to be performed for raw data processing, normalization, and differential gene expression. Finally, we have included a section on studying deep sequencing results in Xenopus, which offers general guidance as to what can be done in this model.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 26%
Student > Ph. D. Student 6 19%
Student > Doctoral Student 5 16%
Student > Bachelor 4 13%
Unknown 8 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 42%
Biochemistry, Genetics and Molecular Biology 5 16%
Medicine and Dentistry 2 6%
Engineering 1 3%
Unknown 10 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 September 2012.
All research outputs
#18,314,922
of 22,678,224 outputs
Outputs from Methods in molecular biology
#7,828
of 13,037 outputs
Outputs of similar age
#128,771
of 168,851 outputs
Outputs of similar age from Methods in molecular biology
#36
of 68 outputs
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So far Altmetric has tracked 13,037 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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