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Mendeley readers
Chapter title |
Targeted Gene Disruption with Engineered Zinc-Finger Nucleases (ZFNs).
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Chapter number | 7 |
Book title |
Xenopus Protocols
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Published in |
Methods in molecular biology, September 2012
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DOI | 10.1007/978-1-61779-992-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-61779-991-4, 978-1-61779-992-1
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Authors |
Young JJ, Harland RM, John J. Young, Richard M. Harland |
Abstract |
Reverse genetics in Xenopus has been limited to knockdown strategies using antisense morpholino oligonucleotides (MOs). Recently, engineered zinc-finger nucleases have been used to induce targeted mutations resulting in null alleles. Zinc-finger nuclease (ZFN) technology has been adapted to induce null mutations in many systems previously refractory to targeted gene inactivation. Here we provide a general protocol for inducing targeted mutations in Xenopus tropicalis using ZFNs, a method to detect resulting mutations, and the steps to generate homozygous mutant embryos. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 6% |
Unknown | 17 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 22% |
Researcher | 4 | 22% |
Professor | 3 | 17% |
Professor > Associate Professor | 2 | 11% |
Other | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 44% |
Agricultural and Biological Sciences | 6 | 33% |
Chemistry | 1 | 6% |
Unknown | 3 | 17% |