Chapter title |
Proteostasis
|
---|---|
Chapter number | 28 |
Book title |
Proteostasis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3756-1_28 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3754-7, 978-1-4939-3756-1
|
Authors |
Poulsen, Esben G, Nielsen, Sofie V, Pietras, Elin J, Johansen, Jens V, Steinhauer, Cornelia, Hartmann-Petersen, Rasmus, Esben G. Poulsen, Sofie V. Nielsen, Elin J. Pietras, Jens V. Johansen, Cornelia Steinhauer, Rasmus Hartmann-Petersen Ph.D., Rasmus Hartmann-Petersen |
Editors |
Rune Matthiesen |
Abstract |
The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function. |
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