Chapter title |
Mesenchymal Stem Cells
|
---|---|
Chapter number | 13 |
Book title |
Mesenchymal Stem Cells
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3584-0_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3582-6, 978-1-4939-3584-0
|
Authors |
Magatti, Marta, Pianta, Stefano, Silini, Antonietta, Parolini, Ornella, Marta Magatti, Stefano Pianta, Antonietta Silini, Ornella Parolini Ph.D., Ornella Parolini |
Editors |
Massimiliano Gnecchi |
Abstract |
During the past several years, the human placenta and in particular the amniotic fetal membrane have attracted much attention as a possible source of cells to be used in cell therapy approaches due to its putative stem cell potential associated with its early embryonic origin and its immunomodulatory potential associated with its role in fetomaternal tolerance.Within the human amniotic membrane, it is possible to isolate two main cell populations: amniotic epithelial cells (from the epithelial layer of the amniotic membrane) and amniotic mesenchymal cells (from the mesenchymal layer of the amniotic membrane). In this chapter, we will describe a method for the isolation of mesenchymal stromal cells (hAMSCs). We will also describe their optimal culture conditions and the phenotypic characterization of cells after passaging. |
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