Chapter title |
GMP-Compliant Expansion of Clinical-Grade Human Mesenchymal Stromal/Stem Cells Using a Closed Hollow Fiber Bioreactor.
|
---|---|
Chapter number | 23 |
Book title |
Mesenchymal Stem Cells
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3584-0_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3582-6, 978-1-4939-3584-0
|
Authors |
Christina Barckhausen, Brent Rice, Stefano Baila, Luc Sensebé, Hubert Schrezenmeier, Philipp Nold, Holger Hackstein, Markus Thomas Rojewski |
Editors |
Massimiliano Gnecchi |
Abstract |
This chapter describes a method for GMP-compliant expansion of human mesenchymal stromal/stem cells (hMSC) from bone marrow aspirates, using the Quantum(®) Cell Expansion System from Terumo BCT. The Quantum system is a functionally closed, automated hollow fiber bioreactor system designed to reproducibly grow cells in either GMP or research laboratory environments. The chapter includes protocols for preparation of media, setup of the Quantum system, coating of the hollow fiber bioreactor, as well as loading, feeding, and harvesting of cells. We suggest a panel of quality controls for the starting material, the interim product, as well as the final product. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 2% |
Unknown | 41 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 26% |
Student > Master | 6 | 14% |
Student > Ph. D. Student | 6 | 14% |
Student > Bachelor | 4 | 10% |
Professor | 3 | 7% |
Other | 4 | 10% |
Unknown | 8 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 9 | 21% |
Biochemistry, Genetics and Molecular Biology | 8 | 19% |
Chemical Engineering | 6 | 14% |
Engineering | 5 | 12% |
Agricultural and Biological Sciences | 2 | 5% |
Other | 1 | 2% |
Unknown | 11 | 26% |