Chapter title |
Construction and Analysis of Randomized Protein-Encoding Libraries Using Error-Prone PCR
|
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Chapter number | 15 |
Book title |
Protein Nanotechnology
|
Published in |
Methods in molecular biology, January 2013
|
DOI | 10.1007/978-1-62703-354-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-62703-353-4, 978-1-62703-354-1, 978-1-62703-353-4, 978-1-62703-354-1
|
Authors |
Paulina Hanson-Manful, Wayne M. Patrick, Hanson-Manful, Paulina, Patrick, Wayne M, Patrick, Wayne M. |
Abstract |
In contrast to site-directed mutagenesis and rational design, directed evolution harnesses Darwinian principles to identify proteins with new or improved properties. The critical first steps in a directed evolution experiment are as follows: (a) to introduce random diversity into the gene of interest and (b) to capture that diversity by cloning the resulting population of molecules into a suitable expression vector, en bloc. Error-prone PCR (epPCR) is a common method for introducing random mutations into a gene. In this chapter, we describe detailed protocols for epPCR and for the construction of large, maximally diverse libraries of cloned variants. We also describe the utility of an online program, PEDEL-AA, for analyzing the compositions of epPCR libraries. The methods described here were used to construct several libraries in our laboratory. A side-by-side comparison of the results is used to show that, ultimately, epPCR is a highly stochastic process. |
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