Intrinsically Disordered Proteins

Table of Contents

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   Book Overview
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   Chapter 1 Disorder for Dummies: Functional Mutagenesis of Transient Helical Segments in Disordered Proteins
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   Chapter 2 Computational Prediction of Intrinsic Disorder in Protein Sequences with the disCoP Meta-predictor
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   Chapter 3 Computational Prediction of Disordered Protein Motifs Using SLiMSuite
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   Chapter 4 How to Annotate and Submit a Short Linear Motif to the Eukaryotic Linear Motif Resource
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   Chapter 5 Analyzing the Sequences of Intrinsically Disordered Regions with CIDER and localCIDER
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   Chapter 6 Exploring Protein Intrinsic Disorder with MobiDB
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   Chapter 7 An Easy Protocol for Evolutionary Analysis of Intrinsically Disordered Proteins
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   Chapter 8 Expression and Purification of an Intrinsically Disordered Protein
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    Chapter 9 Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricks
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    Chapter 10 Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tracts and Beyond
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    Chapter 11 Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy
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    Chapter 12 Structural Analyses of Intrinsically Disordered Proteins by Small-Angle X-Ray Scattering
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    Chapter 13 Determining Rg of IDPs from SAXS Data
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    Chapter 14 Obtaining Hydrodynamic Radii of Intrinsically Disordered Protein Ensembles by Pulsed Field Gradient NMR Measurements
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    Chapter 15 Quantitative Protein Disorder Assessment Using NMR Chemical Shifts
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    Chapter 16 Determination of pKa Values in Intrinsically Disordered Proteins
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    Chapter 17 Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates
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    Chapter 18 Predicting Conformational Properties of Intrinsically Disordered Proteins from Sequence
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    Chapter 19 Enhanced Molecular Dynamics Simulations of Intrinsically Disordered Proteins
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    Chapter 20 Computational Protocol for Determining Conformational Ensembles of Intrinsically Disordered Proteins
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    Chapter 21 Computing, Analyzing, and Comparing the Radius of Gyration and Hydrodynamic Radius in Conformational Ensembles of Intrinsically Disordered Proteins
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    Chapter 22 Binding Thermodynamics to Intrinsically Disordered Protein Domains
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    Chapter 23 Analysis of Multivalent IDP Interactions: Stoichiometry, Affinity, and Local Concentration Effect Measurements
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    Chapter 24 NMR Lineshape Analysis of Intrinsically Disordered Protein Interactions
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    Chapter 25 Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers
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    Chapter 26 Determining the Protective Activity of IDPs Under Partial Dehydration and Freeze-Thaw Conditions
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    Chapter 27 Screening Intrinsically Disordered Regions for Short Linear Binding Motifs
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    Chapter 28 Probing IDP Interactions with Membranes by Fluorescence Spectroscopy
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    Chapter 29 Protocol for Investigating the Interactions Between Intrinsically Disordered Proteins and Membranes by Neutron Reflectometry
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    Chapter 30 Interactions of IDPs with Membranes Using Dark-State Exchange NMR Spectroscopy
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    Chapter 31 Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance
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    Chapter 32 Measuring and Analyzing Binding Kinetics of Coupled Folding and Binding Reactions Under Pseudo-First-Order Conditions
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    Chapter 33 Understanding Binding-Induced Folding by Temperature Jump
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    Chapter 34 Determining Binding Kinetics of Intrinsically Disordered Proteins by NMR Spectroscopy
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    Chapter 35 Determination of Protein Phase Diagrams by Centrifugation
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    Chapter 36 In Vitro Transition Temperature Measurement of Phase-Separating Proteins by Microscopy
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    Chapter 37 Walking Along a Protein Phase Diagram to Determine Coexistence Points by Static Light Scattering
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    Chapter 38 Expression and Purification of Intrinsically Disordered Aβ Peptide and Setup of Reproducible Aggregation Kinetics Experiment
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    Chapter 39 Measuring Interactions Between Tau and Aggregation Inducers with Single-Molecule Förster Resonance Energy Transfer
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    Chapter 40 Detection of Multisite Phosphorylation of Intrinsically Disordered Proteins Using Phos-tag SDS-PAGE
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    Chapter 41 Multiple Site-Specific Phosphorylation of IDPs Monitored by NMR
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    Chapter 42 Detection of Multisite Phosphorylation of Intrinsically Disordered Proteins Using Quantitative Mass-Spectrometry
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    Chapter 43 Targeting an Intrinsically Disordered Protein by Covalent Modification
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    Chapter 44 Recording In-Cell NMR-Spectra in Living Mammalian Cells
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    Chapter 45 In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells
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    Chapter 46 Analyzing IDPs in Interactomes