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Pollen and Pollen Tube Biology

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Cover of 'Pollen and Pollen Tube Biology'

Table of Contents

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    Book Overview
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    Chapter 1 Pollen Grain Counting Using a Cell Counter
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    Chapter 2 A Toolkit for Teasing Apart the Early Stages of Pollen–Stigma Interactions in Arabidopsis thaliana
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    Chapter 3 Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-Style Penetration
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    Chapter 4 Isolation of the Pistil-Stimulated Pollen Tube Secretome
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    Chapter 5 Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil
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    Chapter 6 Semi-In Vivo Assay for Pollen Tube Attraction
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    Chapter 7 Isolation and Cloning of Suppressor Mutants with Improved Pollen Fertility
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    Chapter 8 Workflow to Characterize Mutants with Reproductive Defects
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    Chapter 9 Internally Controlled Methods to Quantify Pollen Tube Growth and Penetration Defects in Arabidopsis thaliana
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    Chapter 10 Arabidopsis thaliana Pollen Tube Culture for Multi-omics Studies
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    Chapter 11 Large-Scale Analysis of Pollen Viability and Oxidative Level Using H2DCFDA-Staining Coupled with Flow Cytometry
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    Chapter 12 Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual Staining
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    Chapter 13 Galvanotropic Chamber for Controlled Reorientation of Pollen Tube Growth and Simultaneous Confocal Imaging of Intracellular Dynamics
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    Chapter 14 Analyzing Intracellular Gradients in Pollen Tubes
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    Chapter 15 Silicone Chambers for Pollen Tube Imaging in Microstructured In Vitro Environments
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    Chapter 16 Analyzing the Impact of Protein Overexpression on Ca2+ Dynamics and Development in Tobacco Pollen Tubes
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    Chapter 17 Imaging and Analysis of the Content of Callose, Pectin, and Cellulose in the Cell Wall of Arabidopsis Pollen Tubes Grown In Vitro
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    Chapter 18 Characterization of Growth Behavior and the Resulting Forces Applied by Pollen Tubes in a 3D Matrix
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    Chapter 19 Flow Chamber Assay to Image the Response of FRET-Based Nanosensors in Pollen Tubes to Changes in Medium Composition
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    Chapter 20 Quantification of Mechanical Forces and Physiological Processes Involved in Pollen Tube Growth Using Microfluidics and Microrobotics
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    Chapter 21 Measuring Exocytosis Rate in Arabidopsis Pollen Tubes Using Corrected Fluorescence Recovery After Photoconversion (cFRAPc) Technique
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    Chapter 22 Testing Pollen Tube Proteins for In Vivo Binding to Phosphatidic Acid by n-Butanol Treatment and Confocal Microscopy
Attention for Chapter 15: Silicone Chambers for Pollen Tube Imaging in Microstructured In Vitro Environments
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Chapter title
Silicone Chambers for Pollen Tube Imaging in Microstructured In Vitro Environments
Chapter number 15
Book title
Pollen and Pollen Tube Biology
Published by
Humana, New York, NY, June 2020
DOI 10.1007/978-1-0716-0672-8_15
Pubmed ID
Book ISBNs
978-1-07-160671-1, 978-1-07-160672-8

Hana Bertrand-Rakusová, Youssef Chebli, Anja Geitmann

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