Chapter title |
Synthetic mRNA
|
---|---|
Chapter number | 19 |
Book title |
Synthetic mRNA
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3625-0_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3623-6, 978-1-4939-3625-0
|
Authors |
Koh, Sarene, Shimasaki, Noriko, Bertoletti, Antonio, Sarene Koh, Noriko Shimasaki, Antonio Bertoletti |
Abstract |
Autologous T lymphocytes genetically modified to express T cell receptors or chimeric antigen receptors have shown great promise in the treatment of several cancers, including melanoma and leukemia. In addition to tumor-associated antigens and tumor-specific neoantigens, tumors expressing viral peptides can also be recognized by specific T cells and are attractive targets for cell therapy. Hepatocellular carcinoma cells often have hepatitis B virus DNA integration and can be targeted by hepatitis B virus-specific T cells. Here, we describe a method to engineer hepatitis B virus-specific T cell receptors in primary human T lymphocytes based on electroporation of hepatitis B virus T cell receptor messenger RNA. This method can be extended to a large scale therapeutic T cell production following current good manufacturing practice compliance and is applicable to the redirection of T lymphocytes with T cell receptors of other virus specificities such as Epstein-Barr virus, cytomegalovirus, and chimeric receptors specific for other antigens expressed on cancer cells. |
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