| Chapter title |
Synthetic Capped mRNAs for Cap-Specific Photo-Cross-Linking Experiments
|
|---|---|
| Chapter number | 2 |
| Book title |
Synthetic mRNA
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3625-0_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3623-6, 978-1-4939-3625-0
|
| Authors |
Joanna Kowalska, Franck Martin, Jacek Jemielity, Kowalska, Joanna, Martin, Franck, Jemielity, Jacek |
| Abstract |
The 7-methylguanosine triphosphate cap present at the 5' ends of eukaryotic mRNAs plays numerous roles in mRNA expression and metabolism. The identification and studies on cap-binding partners can be significantly advanced using tailored chemical tools such as synthetic cap analogues or RNAs carrying modified cap structures. Here we provide protocols for the production of mRNAs specifically labeled within the 5' cap with a nucleoside capable of being photo-activated, either 6-thioguanosine or 7-methyl-6-thioguanosine, which can be used in photo-cross-linking experiments to identify or characterize cap-binding biomolecules. We also describe a protocol for the cross-linking experiments with capped RNAs to map histone H4 cap-binding pocket. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 40% |
| Student > Bachelor | 1 | 20% |
| Unknown | 2 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Agricultural and Biological Sciences | 1 | 20% |
| Chemistry | 1 | 20% |
| Unknown | 2 | 40% |