Chapter title |
Gene Inactivation in Coxiella burnetii.
|
---|---|
Chapter number | 19 |
Book title |
Host-Bacteria Interactions
|
Published in |
Methods in molecular biology, July 2014
|
DOI | 10.1007/978-1-4939-1261-2_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1260-5, 978-1-4939-1261-2
|
Authors |
Beare PA, Heinzen RA, Paul A. Beare, Robert A. Heinzen, Beare, Paul A., Heinzen, Robert A. |
Editors |
Annette C. Vergunst, David O'Callaghan |
Abstract |
Coxiella burnetii, the agent of human Q fever, is a zoonotic bacterial pathogen with a worldwide distribution. Owing to an historic lack of methods for genetic manipulation, virulence factors deployed by this bacterium for disease pathogenesis are poorly understood. However, the recent advance of host cell-free (axenic) growth of C. burnetii has coincided with development of several new genetic technologies including site-specific and random transposon systems, shuttle vectors, and an inducible gene expression system. We have recently added two methods for targeted gene inactivation to the expanding C. burnetii genetics toolbox. Here, we describe a "loop in/loop out" gene inactivation system for C. burnetii. This procedure allows for generation of site-directed mutants in approximately 10 weeks and has been used by our laboratory to generate more than 50 individual C. burnetii mutants. The collection of C. burnetii genetic tools now allows for conventional mutation and complementation strategies to define virulence factors. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 21% |
Student > Ph. D. Student | 3 | 21% |
Lecturer > Senior Lecturer | 1 | 7% |
Student > Doctoral Student | 1 | 7% |
Student > Bachelor | 1 | 7% |
Other | 2 | 14% |
Unknown | 3 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 4 | 29% |
Agricultural and Biological Sciences | 3 | 21% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Medicine and Dentistry | 2 | 14% |
Unknown | 3 | 21% |