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In Situ Hybridization Protocols

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Table of Contents

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    Book Overview
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    Chapter 1 Guidelines for the Optimization and Validation of In Situ Hybridization
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    Chapter 2 Overcoming Autofluorescence (AF) and Tissue Variation in Image Analysis of In Situ Hybridization
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    Chapter 3 Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboratories
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    Chapter 4 Fluorescent In Situ Hybridization Using Oligonucleotide-Based Probes
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    Chapter 5 Visualizing Genome Reorganization Using 3D DNA FISH
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    Chapter 6 MicroRNA In Situ Hybridization in Paraffin-Embedded Cultured Cells
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    Chapter 7 Multiplexed Detection and Analysis of Low-Abundance Long Noncoding RNA Using RNAscope™ in Cultured Cells
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    Chapter 8 Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR Flow Cytometry (v3.0)
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    Chapter 9 Multiplexed Quantitative In Situ Hybridization for Mammalian Cells on a Slide: qHCR and dHCR Imaging (v3.0)
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    Chapter 10 Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution Within Whole-Mount Vertebrate Embryos: qHCR and dHCR Imaging (v3.0)
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    Chapter 11 Hybridization Chain Reaction for Quantitative and Multiplex Imaging of Gene Expression in Amphioxus Embryos and Adult Tissues
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    Chapter 12 RNAscope™ Multiplex Detection in Zebrafish
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    Chapter 13 Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues Using RNAscope ®
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    Chapter 14 Automated ISH for Validated Histological Mapping of Lowly Expressed Genes
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    Chapter 15 Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping
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    Chapter 16 Automated Co-in Situ Hybridization and Immunofluorescence Using Archival Tumor Tissue
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    Chapter 17 Automated Five-Color Multiplex Co-detection of MicroRNA and Protein Expression in Fixed Tissue Specimens
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    Chapter 18 Mixed Multiplex Staining: Automated RNAscope™ and OPAL™ for Multiple Targets
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    Chapter 19 Simultaneous Visualization of RNA and Protein Expression in Tissue Using a Combined RNAscope™ In Situ Hybridization and Immunofluorescence Protocol
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    Chapter 20 In Situ Sequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections
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    Chapter 21 GeoMx™ RNA Assay: High Multiplex, Digital, Spatial Analysis of RNA in FFPE Tissue
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    Chapter 22 In Situ Point Mutation Detection in FFPE Colorectal Cancers Using the BaseScope Assay
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    Chapter 23 Using In Situ Padlock Probe Technology to Detect mRNA Splice Variants in Tumor Cells
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    Chapter 24 Automated One-Double-Z Pair BaseScope™ for CircRNA In Situ Hybridization
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Title
In Situ Hybridization Protocols
Published by
Springer US, July 2020
DOI 10.1007/978-1-0716-0623-0
ISBNs
978-1-07-160622-3, 978-1-07-160623-0
Editors

Nielsen, Boye Schnack, Jones, Julia

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