Trypanosomatids

Table of Contents

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   Book Overview
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   Chapter 1 Field Isolation and Cultivation of Trypanosomatids from Insects
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   Chapter 2 Culturing and Transfection of Pleomorphic Trypanosoma brucei
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   Chapter 3 In Vitro Culture for Differentiation Simulation of Leishmania spp.
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   Chapter 4 Tsetse Fly Transmission Studies of African Trypanosomes
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   Chapter 5 Infecting Triatomines with Trypanosomes
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   Chapter 6 High-Throughput Sequencing for Trypanosome Transcriptome Characterization
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   Chapter 7 Polysome Profiling and Metabolic Labeling Methods to Measure Translation in Trypanosoma brucei
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   Chapter 8 Immunoprecipitation for the Analysis of Macromolecular Complexes in Trypanosoma cruzi
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    Chapter 9 Analysis of the In Vivo Translation Process in Trypanosoma cruzi Using Ribosome Profiling
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    Chapter 10 Proteome-Wide Quantitative Phosphoproteomic Analysis of Trypanosoma brucei Insect and Mammalian Life Cycle Stages
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    Chapter 11 Genome-Wide Proteomics and Phosphoproteomics Analysis of Trypanosoma cruzi During Differentiation
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    Chapter 12 Genome-Wide Proteomics and Phosphoproteomics Analysis of Leishmania spp. During Differentiation
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    Chapter 13 CRISPR/Cas9 Technology Applied to the Study of Proteins Involved in Calcium Signaling in Trypanosoma cruzi
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    Chapter 14 Application of CRISPR/Cas9-Mediated Genome Editing in Leishmania
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    Chapter 15 Next-Generation Analysis of Trypanosomatid Genome Stability and Instability
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    Chapter 16 Transcription Factor Analysis in Trypanosomatids
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    Chapter 17 Identifying Trypanosome Protein–RNA Interactions Using RIP-Seq
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    Chapter 18 The Tethering Assay: A Simple Method for the Characterization of mRNA-Fate Regulators
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    Chapter 19 RNA-Binding Proteins and Their Targets in Trypanosoma brucei: Single Nucleotide Resolution Using iCLIP and iCLAP
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    Chapter 20 In Vivo Tethering System to Isolate RNA-Binding Proteins Regulating mRNA Decay in Leishmania
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    Chapter 21 Forward Genetics in African Trypanosomes
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    Chapter 22 Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts
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    Chapter 23 Light Microscopy in Trypanosomes: Use of Fluorescent Proteins and Tags
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    Chapter 24 ImageJ for Partially and Fully Automated Analysis of Trypanosome Micrographs
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    Chapter 25 Motility Analysis of Trypanosomatids
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    Chapter 26 Electron Microscopy Techniques Applied to Symbiont-Harboring Trypanosomatids: The Association of the Bacterium with Host Organelles
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    Chapter 27 Airyscan Superresolution Microscopy to Study Trypanosomatid Cell Biology
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    Chapter 28 All You Ever Wanted to Know About APOL1 and TLFs and Did Not Dare Ask
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    Chapter 29 Isolation of Leishmania Promastigote Flagella
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    Chapter 30 Gel-Based Methods for the Investigation of Signal Transduction Pathways in Trypanosoma brucei
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    Chapter 31 Methods to Investigate Signal Transduction Pathways in Trypanosoma cruzi: Cyclic Nucleotide Phosphodiesterases Assay Protocols
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    Chapter 32 Methods for the Investigation of Trypanosoma cruzi Amastigote Proliferation in Mammalian Host Cells
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    Chapter 33 Isolation of Extracellular Vesicles from Leishmania spp.
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    Chapter 34 2D Gel Electrophoresis Analysis of Leishmania Proteomes
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    Chapter 35 Analysis of the Physiological and Metabolic State of Leishmania Using Heavy Water Labeling
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    Chapter 36 A Scalable Purification Method for Mitochondria from Trypanosoma brucei
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    Chapter 37 Isolation of Glycosomes from Trypanosoma cruzi
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    Chapter 38 Sorting the Muck from the Brass: Analysis of Protein Complexes and Cell Lysates
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    Chapter 39 Measurement of Energy States of the Trypanosomatid Mitochondrion
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    Chapter 40 Isolation and Characterization of Acidocalcisomes from Trypanosomatids
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    Chapter 41 Metabolic Control Analysis for Drug Target Prioritization in Trypanosomatids
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    Chapter 42 Establishment of a Standardized Vaccine Protocol for the Analysis of Protective Immune Responses During Experimental Trypanosome Infections in Mice
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    Chapter 43 Isolation of Trypanosoma brucei brucei Infection-Derived Splenic Marginal Zone B Cells Based on CD1dHigh/B220High Surface Expression in a Two-Step MACS-FACS Approach
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    Chapter 44 Cellular Markers for the Identification of Chemoresistant Isolates in Leishmania
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    Chapter 45 In Vitro Image-Based Assay for Trypanosoma cruzi Intracellular Forms
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    Chapter 46 In Vitro Drug Efficacy Testing Against Trypanosoma brucei
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    Chapter 47 In Vitro Growth Inhibition Assays of Leishmania spp.
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    Chapter 48 In Vivo Bioluminescence Imaging to Assess Compound Efficacy Against Trypanosoma brucei