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Ribozymes

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Cover of 'Ribozymes'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction
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    Chapter 2 Characterization of Hammerhead Ribozyme Reactions
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    Chapter 3 Mechanistic Analysis of the Hepatitis Delta Virus (HDV) Ribozyme: Methods for RNA Preparation, Structure Mapping, Solvent Isotope Effects, and Co-transcriptional Cleavage
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    Chapter 4 Kinetic Characterization of Hairpin Ribozyme Variants
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    Chapter 5 Characterization of RNase P RNA Activity
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    Chapter 6 Group I Intron Ribozymes
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    Chapter 7 Kinetic Characterization of Group II Intron Folding and Splicing
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    Chapter 8 Mechanism and Distribution of glmS Ribozymes
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    Chapter 9 Structure-Based Search and In Vitro Analysis of Self-Cleaving Ribozymes
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    Chapter 10 Discovery of RNA Motifs Using a Computational Pipeline that Allows Insertions in Paired Regions and Filtering of Candidate Sequences.
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    Chapter 11 Crystallographic Analysis of Small Ribozymes and Riboswitches
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    Chapter 12 Functional Dynamics of RNA Ribozymes Studied by NMR Spectroscopy
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    Chapter 13 Ribozymes
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    Chapter 14 Ribozymes
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    Chapter 15 Single Molecule FRET Characterization of Large Ribozyme Folding
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    Chapter 16 Metal Ion–RNA Interactions Studied via Multinuclear NMR
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    Chapter 17 Analysis of Catalytic RNA Structure and Function by Nucleotide Analog Interference Mapping
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    Chapter 18 In Vitro Selection of Metal Ion-Selective DNAzymes
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    Chapter 19 Selecting Allosteric Ribozymes
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    Chapter 20 Screening Effective Target Sites on mRNA: A Ribozyme Library Approach
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    Chapter 21 A Computational Approach to Predict Suitable Target Sites for trans -Acting Minimal Hammerhead Ribozymes
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    Chapter 22 Targeting mRNAs by Engineered Sequence-Specific RNase P Ribozymes
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    Chapter 23 Target-Induced SOFA-HDV Ribozyme
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    Chapter 24 Ribozyme-Mediated Trans Insertion-Splicing into Target RNAs
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    Chapter 25 Developing Fluorogenic RNA-Cleaving DNAzymes for Biosensing Applications
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    Chapter 26 Ribozymes
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    Chapter 27 Rational design and tuning of ribozyme-based devices.
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    Chapter 28 In vivo screening of ligand-dependent hammerhead ribozymes.
  30. Altmetric Badge
    Chapter 29 Flexizymes as a tRNA Acylation Tool Facilitating Genetic Code Reprogramming
Attention for Chapter 27: Rational design and tuning of ribozyme-based devices.
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Chapter title
Rational design and tuning of ribozyme-based devices.
Chapter number 27
Book title
Ribozymes
Published in
Methods in molecular biology, February 2012
DOI 10.1007/978-1-61779-545-9_27
Pubmed ID
Book ISBNs
978-1-61779-544-2, 978-1-61779-545-9
Authors

Liang JC, Smolke CD, Joe C. Liang, Christina D. Smolke, Liang, Joe C., Smolke, Christina D.

Abstract

A synthetic gene-regulatory device platform was described by modularly assembling three RNA components encoding distinct functions of sensing, transmission, and actuation. The molecular binding at the sensor component is translated by the transmitter component through a strand-displacement event to modulate activity of the actuator component, which then interacts with cellular transcriptional machinery to affect gene expression levels. Here, we provide some general guidelines on linking RNA components to construct gene-regulatory devices and strategies to tune device regulatory activities through an RNA folding -program for specific cellular applications.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 12%
Unknown 15 88%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 41%
Researcher 5 29%
Professor 2 12%
Professor > Associate Professor 2 12%
Other 1 6%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 53%
Biochemistry, Genetics and Molecular Biology 3 18%
Engineering 2 12%
Chemistry 1 6%
Computer Science 1 6%
Other 0 0%
Unknown 1 6%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 May 2012.
All research outputs
#18,308,895
of 22,668,244 outputs
Outputs from Methods in molecular biology
#7,828
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Outputs of similar age
#197,661
of 248,345 outputs
Outputs of similar age from Methods in molecular biology
#315
of 456 outputs
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So far Altmetric has tracked 13,025 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 456 others from the same source and published within six weeks on either side of this one. This one is in the 10th percentile – i.e., 10% of its contemporaries scored the same or lower than it.