| Chapter title |
Detection and Quantification of Protein Aggregates in Plants.
|
|---|---|
| Chapter number | 15 |
| Book title |
Plant Proteostasis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3759-2_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3757-8, 978-1-4939-3759-2
|
| Authors |
Marc Planas-Marquès, Saul Lema A., Núria S. Coll |
| Editors |
L. Maria Lois, Rune Matthiesen |
| Abstract |
Plants are constantly exposed to a complex and changing environment that challenges their cellular homeostasis. Stress responses triggered as a consequence of unfavorable conditions result in increased protein aggregate formation at the cellular level. When the formation of misfolded proteins surpasses the capacity of the cell to remove them, insoluble protein aggregates accumulate. In the animal field, an enormous effort is being placed to uncover the mechanisms regulating aggregate formation because of its implications in many important human diseases. Because of its importance for cellular functionality and fitness, it is equally important to expand plant research in this field. Here, we describe a cell fractionation-based method to obtain very pure insoluble protein aggregate fractions that can be subsequently semiquantified using image analysis. This method can be used as a first step to evaluate whether a particular condition results in an alteration of protein aggregate formation levels. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 15% |
| Student > Ph. D. Student | 3 | 15% |
| Student > Postgraduate | 2 | 10% |
| Other | 1 | 5% |
| Researcher | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 9 | 45% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 30% |
| Biochemistry, Genetics and Molecular Biology | 4 | 20% |
| Unknown | 10 | 50% |