Chapter title |
UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods
|
---|---|
Chapter number | 109 |
Book title |
Biofuels from Algae
|
Published in |
Methods in molecular biology, November 2017
|
DOI | 10.1007/7651_2017_109 |
Pubmed ID | |
Book ISBNs |
978-1-4939-9415-1, 978-1-4939-9416-8
|
Authors |
Tuulikki Seppänen-Laakso, Heli Nygren, Heiko Rischer, Seppänen-Laakso, Tuulikki, Nygren, Heli, Rischer, Heiko |
Abstract |
Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps. |
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