| Chapter title |
Light-inducible gene regulation with engineered zinc finger proteins.
|
|---|---|
| Chapter number | 7 |
| Book title |
Photoswitching Proteins
|
| Published in |
Methods in molecular biology, March 2014
|
| DOI | 10.1007/978-1-4939-0470-9_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-0469-3, 978-1-4939-0470-9
|
| Authors |
Polstein LR, Gersbach CA, Lauren R. Polstein, Charles A. Gersbach Ph.D., Polstein, Lauren R., Gersbach, Charles A., Charles A. Gersbach |
| Editors |
Sidney Cambridge |
| Abstract |
The coupling of light-inducible protein-protein interactions with gene regulation systems has enabled the control of gene expression with light. In particular, heterodimer protein pairs from plants can be used to engineer a gene regulation system in mammalian cells that is reversible, repeatable, tunable, controllable in a spatiotemporal manner, and targetable to any DNA sequence. This system, Light-Inducible Transcription using Engineered Zinc finger proteins (LITEZ), is based on the blue light-induced interaction of GIGANTEA and the LOV domain of FKF1 that drives the localization of a transcriptional activator to the DNA-binding site of a highly customizable engineered zinc finger protein. This chapter provides methods for modifying LITEZ to target new DNA sequences, engineering a programmable LED array to illuminate cell cultures, and using the modified LITEZ system to achieve spatiotemporal control of transgene expression in mammalian cells. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 1 | 4% |
| Unknown | 26 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 19% |
| Researcher | 3 | 11% |
| Student > Master | 3 | 11% |
| Student > Bachelor | 3 | 11% |
| Student > Doctoral Student | 2 | 7% |
| Other | 7 | 26% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 33% |
| Agricultural and Biological Sciences | 8 | 30% |
| Unspecified | 1 | 4% |
| Social Sciences | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 4 | 15% |