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Photoswitching Proteins

Overview of attention for book
Cover of 'Photoswitching Proteins'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Photoinduced damage resulting from fluorescence imaging of live cells.
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    Chapter 2 Modification of purified proteins with photochemical protection compounds for high-resolution photoactivation of protein function in vitro and in vivo.
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    Chapter 3 Optochemical activation of kinase function in live cells.
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    Chapter 4 Photoswitching of cell surface receptors using tethered ligands.
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    Chapter 5 Photocontrol of AMPA receptors with a photochromic ligand.
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    Chapter 6 Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy.
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    Chapter 7 Light-inducible gene regulation with engineered zinc finger proteins.
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    Chapter 8 Manipulation of plasma membrane phosphoinositides using photoinduced protein-protein interactions.
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    Chapter 9 A roadmap to applying optogenetics in neuroscience.
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    Chapter 10 Salvaging ruins: reverting blind retinas into functional visual sensors.
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    Chapter 11 Photoactivated adenylyl cyclases as optogenetic modulators of neuronal activity.
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    Chapter 12 Structural basis of photoswitching in fluorescent proteins.
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    Chapter 13 Using Photoactivatable GFP to Track Axonal Transport Kinetics.
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    Chapter 14 In Vivo Cell Tracking Using PhOTO Zebrafish.
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    Chapter 15 In Vivo Optogenetics for Light-Induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein.
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    Chapter 16 Photoactivatable Fluorescent Proteins for Super-resolution Microscopy.
  18. Altmetric Badge
    Chapter 17 pcSOFI as a Smart Label-Based Superresolution Microscopy Technique.
Attention for Chapter 13: Using Photoactivatable GFP to Track Axonal Transport Kinetics.
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Chapter title
Using Photoactivatable GFP to Track Axonal Transport Kinetics.
Chapter number 13
Book title
Photoswitching Proteins
Published in
Methods in molecular biology, March 2014
DOI 10.1007/978-1-4939-0470-9_13
Pubmed ID
24718803
Book ISBNs
978-1-4939-0469-3, 978-1-4939-0470-9
Authors

Archan Ganguly Ph.D., Subhojit Roy M.D., Ph.D., Archan Ganguly, Subhojit Roy

Editors

Sidney Cambridge

Abstract

The advent of photoactivatable tools has revolutionized imaging of dynamic cellular processes. One such application is to visualize axonal transport-an intricate and dynamic process by which proteins and other macromolecules are conveyed from their sites of synthesis in the cell bodies to their destinations within axons and synapses. High-quality dynamic imaging of axonal transport using photoactivatable vectors can now be routinely performed using epifluorescence microscopes and CCD cameras that are standard in most laboratories, yet this is largely underutilized. Here we describe detailed protocols for imaging cargoes moving in fast and slow axonal transport in axons of cultured hippocampal neurons.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Professor 2 18%
Professor > Associate Professor 2 18%
Student > Ph. D. Student 2 18%
Student > Bachelor 1 9%
Researcher 1 9%
Other 1 9%
Unknown 2 18%
Readers by discipline Count As %
Neuroscience 6 55%
Agricultural and Biological Sciences 2 18%
Biochemistry, Genetics and Molecular Biology 1 9%
Medicine and Dentistry 1 9%
Unknown 1 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 April 2016.
All research outputs
#20,322,106
of 22,865,319 outputs
Outputs from Methods in molecular biology
#9,916
of 13,127 outputs
Outputs of similar age
#191,590
of 223,568 outputs
Outputs of similar age from Methods in molecular biology
#93
of 156 outputs
Altmetric has tracked 22,865,319 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 156 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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