Chapter title |
Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells.
|
---|---|
Chapter number | 347 |
Book title |
Stem Cell Heterogeneity
|
Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/7651_2016_347 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6549-6, 978-1-4939-6550-2
|
Authors |
Sharat Warrier, Mina Popovic, Margot Van der Jeught, Björn Heindryckx, Warrier, Sharat, Popovic, Mina, Jeught, Margot Van der, Heindryckx, Björn |
Abstract |
Mouse embryonic stem cells are known to represent the naïve state of pluripotency, while human embryonic stem cells typically represented the primed state of pluripotency, characterized by a higher drift toward differentiation and some other disadvantages. Here we describe an efficient method for rapid, transgene free induction of the naïve pluripotent state in human by applying a novel combination of small molecules and growth factors in the culture medium (2i, LIF, basic fibroblast growth factor, ascorbic acid, and forskolin). Conversion of primed human embryonic stem cells towards the naive pluripotent state should be confirmed by a detailed characterization of the cells, as described in this chapter. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 5 | 28% |
Researcher | 4 | 22% |
Student > Bachelor | 3 | 17% |
Student > Ph. D. Student | 1 | 6% |
Other | 1 | 6% |
Other | 0 | 0% |
Unknown | 4 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 33% |
Agricultural and Biological Sciences | 3 | 17% |
Medicine and Dentistry | 2 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Neuroscience | 1 | 6% |
Other | 0 | 0% |
Unknown | 5 | 28% |