Chapter title |
Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells
|
---|---|
Chapter number | 361 |
Book title |
Stem Cell Heterogeneity
|
Published in |
Methods in molecular biology, June 2016
|
DOI | 10.1007/7651_2016_361 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6549-6, 978-1-4939-6550-2
|
Authors |
Krzysztof Szade, Karolina Bukowska-Strakova, Monika Zukowska, Alicja Jozkowicz, Józef Dulak, Szade, Krzysztof, Bukowska-Strakova, Karolina, Zukowska, Monika, Jozkowicz, Alicja, Dulak, Józef |
Abstract |
Hematopoietic stem cells (HSC) act as paradigmatic tissue-specific adult stem cells. While they are quiescent in steady-state conditions, they enter the cell cycle and proliferate in stress conditions and during tissue regeneration. Therefore, analysis of cell cycle status of HSC is crucial for understanding their biology. However, due to low number of HSC in tissue and need to use many surface markers for their identification, analysis of their cycle status is technically complicated. Here, we presented our simple strategy to analyze cell cycle of strictly defined LKS CD48(-)CD150(+)CD34(-) HSC, together with Ki67 and DAPI staining by flow cytometry. |
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