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Mendeley readers
| Chapter title |
Measurement of Ca(2+)-ATPase Activity (in PMCA and SERCA1).
|
|---|---|
| Chapter number | 21 |
| Book title |
Calcium Signaling Protocols
|
| Published in |
Methods in molecular biology, January 2013
|
| DOI | 10.1007/978-1-62703-086-1_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-085-4, 978-1-62703-086-1
|
| Authors |
Danuta Kosk-Kosicka |
| Abstract |
Ca(2+)-ATP pumps (those on the plasma membrane; PMCA and sarcoplasmic reticulum; SERCA1) have an important role to play in the regulation of intracellular calcium concentrations. In this chapter, three preparations, two membranes and a purified enzyme, best suited for studies of Ca(2+)-ATPase activity are described. The two selected membranes are the human red blood cell (RBC) ghosts, a representative of plasma membranes (PM), and the rabbit skeletal muscle SR, an intracellular membrane. In this protocol, Pi released during the ATPase reaction is subsequently measured colorimetrically as a complex of molybdovanadate. The method is simple (one-step), fast, sensitive, and reliable. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 17% |
| Germany | 1 | 17% |
| Unknown | 4 | 67% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 33% |
| Researcher | 2 | 33% |
| Professor > Associate Professor | 1 | 17% |
| Student > Master | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 50% |
| Computer Science | 1 | 17% |
| Psychology | 1 | 17% |
| Medicine and Dentistry | 1 | 17% |