| Chapter title |
A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 Phosphorylation.
|
|---|---|
| Chapter number | 11 |
| Book title |
mTOR
|
| Published in |
Methods in molecular biology, September 2011
|
| DOI | 10.1007/978-1-61779-430-8_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-429-2, 978-1-61779-430-8
|
| Authors |
Papageorgiou A, Avruch J, Angela Papageorgiou, Joseph Avruch |
| Editors |
Thomas Weichhart |
| Abstract |
Mammalian target of rapamycin (mTOR) is a giant protein kinase that controls cell proliferation, growth, and metabolism. mTOR is regulated by nutrient availability, by mitogens, and by stress, and operates through two independently regulated hetero-oligomeric complexes. We have attempted to identify the cellular components necessary to maintain the activity of mTOR complex 1 (mTORC1), the amino acid-dependent, rapamycin-inhibitable complex, using a whole genome approach involving RNAi-induced depletion of cellular polypeptides. We have used a pancreatic ductal adenocarcinoma (PDAC) cell line, Mia-PaCa for this screen; as with many pancreatic cancers, these cells exhibit constitutive activation of mTORC1. PDAC is the most common form of pancreatic cancer and the 5-year survival rate remains 3-5% despite current nonspecific and targeted therapies. Although rapamycin-related mTOR inhibitors have yet to demonstrate encouraging clinical responses, it is now evident that this class of compounds is capable of only partial mTORC1 inhibition. Identifying previously unappreciated proteins needed for maintenance of mTORC1 activity may provide new targets and lead to the development of beneficial therapies for pancreatic cancer. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 25% |
| Researcher | 3 | 25% |
| Student > Doctoral Student | 1 | 8% |
| Other | 1 | 8% |
| Professor > Associate Professor | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 25% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Engineering | 2 | 17% |
| Medicine and Dentistry | 1 | 8% |
| Computer Science | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |