| Chapter title |
In Vitro Generation of Antigen-Specific T Cells from Induced Pluripotent Stem Cells of Antigen-Specific T Cell Origin.
|
|---|---|
| Chapter number | 6 |
| Book title |
Tumor Immunology
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3338-9_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3336-5, 978-1-4939-3338-9
|
| Authors |
Shin Kaneko, Kaneko, Shin |
| Editors |
Attilio Bondanza, Monica Casucci |
| Abstract |
Induced pluripotent stem (iPS) cells derived from T lymphocyte (T-iPS cells) preserve the T cell receptor (TCR) α and β gene rearrangements identical to the original T cell clone. Re-differentiated CD8 single positive αβ T cells from the T-iPS cells exhibited antigen-specific cytotoxicity, improved proliferative response, and elongation of telomere indicating rejuvenation of antigen specific T cell immunity in vitro. To regenerate antigen specific cytotoxic T lymphocytes (CTL), first, we have optimized a method for reprogramming-resistant CD8 T cell clones into T-iPS cells by using sendaiviral vectors. Second, we have optimized stepwise differentiation methods for inducing hematopoietic progenitor cells, T cell progenitors, and functionally matured CD8 single positive CTL. These protocols provide useful in vitro tools and models both for research of antigen-specific T cell immunotherapy and for research of normal and pathological thymopoiesis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 39% |
| Student > Master | 4 | 11% |
| Student > Ph. D. Student | 4 | 11% |
| Student > Postgraduate | 3 | 8% |
| Student > Bachelor | 3 | 8% |
| Other | 3 | 8% |
| Unknown | 6 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 21% |
| Medicine and Dentistry | 7 | 18% |
| Immunology and Microbiology | 7 | 18% |
| Agricultural and Biological Sciences | 6 | 16% |
| Chemical Engineering | 2 | 5% |
| Other | 1 | 3% |
| Unknown | 7 | 18% |