Neuroproteomics

Chapter 1 Neuroproteomics: The Methods

Chapter 2 Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue

Chapter 3 Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice

Chapter 4 Laser Microdissection for Spatial Proteomics of Postmortem Brain Tissue

Chapter 5 High-pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics

Chapter 6 Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3)

Chapter 7 Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip

Chapter 8 Integrated Immunoprecipitation: Blue Native Gel Electrophoresis—Mass Spectrometry for the Identification of Protein Subcomplexes

Chapter 9 Analysis of Synaptic Protein–Protein Interaction by Cross-linking Mass Spectrometry

Chapter 10 TMT-MS 3 -Enabled Proteomic Quantification of Human IPSC-Derived Neurons

Chapter 11 Data-Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures

Chapter 13 2D-DIGE as a Tool in Neuroproteomics

Chapter 14 Neuropeptidomics of the Mammalian Brain

Chapter 15 MALDI Imaging Mass Spectrometry: Neurochemical Imaging of Proteins and Peptides

Chapter 16 A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder