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The Online Morphology Control and Dynamic Studies on Improving Vitamin B12 Production by Pseudomonas denitrificans with Online Capacitance and Specific Oxygen Consumption Rate

Overview of attention for article published in Applied Biochemistry and Biotechnology, March 2016
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Title
The Online Morphology Control and Dynamic Studies on Improving Vitamin B12 Production by Pseudomonas denitrificans with Online Capacitance and Specific Oxygen Consumption Rate
Published in
Applied Biochemistry and Biotechnology, March 2016
DOI 10.1007/s12010-016-2053-4
Pubmed ID
Authors

Ze-Jian Wang, Hui-lin Shi, Ping Wang

Abstract

The relationship between the morphological character of Pseudomonas denitrificans and vitamin B12 synthesis based on real-time capacitance measurement and online specific oxygen consumption rate (Q O2) control was established for enhancing vitamin B12 production. Results demonstrated that the threshold Q O2 value lower than 2.0 mmol/gDCW/l would greatly stimulate the state transfer from the cell number growth phase to the cell elongation phase and promote rapid vitamin B12 biosynthesis, while the vitamin B12 biosynthesis rate could also be inhibited when the rate of cell's length-to-width ratio (ratio-LW) was higher than 10:1. Furthermore, the optimal morphology controlling strategy was achieved based on online Q O2 control, which increases the appropriate active cell numbers at the former phase, and then control the elongation of ratio-LW no more than 10:1 at the vitamin B12 biosynthesis phase. The maximal vitamin B12 production reached 239.7 mg/l at 168 h, which was improved by 14.7 % compared with the control (208 mg/l). This online controlling strategy would be effectively applied for improving industrial vitamin B12 fermentation.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 25%
Student > Ph. D. Student 5 21%
Researcher 2 8%
Unspecified 1 4%
Student > Master 1 4%
Other 1 4%
Unknown 8 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 38%
Chemistry 3 13%
Agricultural and Biological Sciences 1 4%
Unspecified 1 4%
Immunology and Microbiology 1 4%
Other 1 4%
Unknown 8 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2016.
All research outputs
#15,365,885
of 22,858,915 outputs
Outputs from Applied Biochemistry and Biotechnology
#1,555
of 2,508 outputs
Outputs of similar age
#180,516
of 300,926 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#8
of 33 outputs
Altmetric has tracked 22,858,915 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,508 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 300,926 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.